Fluorescence spectra of 1-Pyrenebutyric acid, concentration dependence and dynamic quenching phenomena
نویسندگان
چکیده
Excitation and emission spectra of PBA were investigated. The concentration dependence of fluorescene was studied showing a maximum intesity at a concentration of 0.039 ± 0.04 mM. 2D-spectroscopy proved the independence of λem on λex. Rayleigh and Raman scattering were observed, showing the shift wavelength νshift = 3800 ± 800 cm−1, which corresponds to the as. st. vibration of water molecules. The dynamic quenching effect of O2 on PBA was studied in two different matrices (silicone, NaOH-solution). The dynamic quenching constant KD = 34000 ± 2100 M−1 was calucalted with kQ = 2.41 · 10 ± 0.16 · 10 M−1s−1. Zürich, the 16.11.2010 J. Ferreiro A. Lauber
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